Colorado State athletic director Joe Parker announced Eustachy's temporary leave in a statement Saturday, just hours before the Rams' home game against Nevada.
"I have asked head coach Larry Eustachy to temporarily step away from the men's basketball program, as he has been placed on administrative leave while we conclude our climate assessment," Parker said. "We are diligently working through this assessment as expeditiously as possible, understanding the importance of a thorough and fair process. No conclusions have been made."
Assistant coach Steve Barnes will coach Colorado State on Saturday night. The school announced Wednesday that it would have conversations with students and staff members about the 62-year-old Eustachy.
It marks the second time in four years that Colorado State officials have interviewed players and others about Eustachy's behavior. A 2013-14 investigation alleged that he created a "culture of fear and intimidation" and emotionally abused his players, according to the 90-page report completed by former Rams athletic director Jack Graham.
Eustachy's contract expires after the 2020-21 season.